Endocrine Disruption and Cytotoxicity of glyphosate and roundup in Human JAr Cells in Vitro

Endocrine Disruption and Cytotoxicity of glyphosate and roundup in Human JAr Cells in Vitro

by GM Watch

Findings of new study need confirmation in animal tests

Roundup is an endocrine disruptor and is toxic to human cells in vitro (tested in culture dishes in the laboratory) at levels permitted in drinking water in Australia, a new study has found.

This is the first study to examine the effects of glyphosate and Roundup on progesterone production by human female cells in an in vitro system that models key aspects of reproduction in women.

Glyphosate alone was less toxic to human cells than glyphosate in a Roundup formulation; both glyphosate and Roundup caused cell death which resulted in decreased progesterone levels – a form of hormone/endocrine disruption. Endocrine disruption did not precede the toxicity to cells but occurred after it. The decreases in progesterone concentrations were caused by reduced numbers of viable cells.

A 24h exposure to a concentration of glyphosate (in Roundup) similar to that recommended as an acceptable level for Australian drinking water caused significant toxicity to the cells in vitro, which supports a call for long-term in vivo (in live animals) studies to characterise the toxicity of Roundup.

The possibility that Roundup has endocrine disrupting activity independent of its ability to kill or disable cells needs further study.

Endocrine disruption and cytotoxicity of glyphosate and roundup in human JAr cells in vitro

Fiona Young, Dao Ho, Danielle Glynn and Vicki Edwards
Department of Medical Biotechnology, Flinders University, Adelaide, South Australia
Integr Pharm Toxicol Genotoxicol, 2015 Volume 1(1): 12-19
doi: 10.15761/IPTG.1000104

Abstract

The toxicity of the active molecule in herbicides has been used to determine safe concentrations, because other components are considered inert. Roundup, which contains the active molecule Glyphosate, was described as an endocrine disrupter because non-cytotoxic concentrations inhibited progesterone synthesis in vitro. Human chorioplacental JAr cells synthesise progesterone, and increase synthesis when stimulated by chorionic gonadotrophin (hCG), or the transduction molecule cAMP.

JAr cells were exposed to two Roundup formulations, and compared with the same concentrations of glyphosate ± cAMP, or ± hCG for 1, 4, 24, 48 or 72h. The surviving viable cells were quantified using an MTT assay, and progesterone was measured in an ELISA. hCG and cAMP stimulated progesterone synthesis by cells in vitro as expected. In contrast to previous reports, JAr cell death preceded decreased progesterone synthesis, and steroidogenesis was unaffected by low, non-cytotoxic concentrations of Roundup or glyphosate. Roundup was more cytotoxic than glyphosate alone; the 24h EC50 was 16mM for glyphosate, but 0.008mM when glyphosate was in a 7.2g/L Roundup formulation. Significant cytotoxicity was caused by glyphosate in Roundup (p<0.01) after 24h, and cytotoxicity was observed in vitro after exposure to a range of concentrations comparable to the Australian Drinking Water Guidelines.

Endocrine disruption effects were secondary to cytotoxicity. Roundup was more cytotoxic than the same concentration of glyphosate alone, indicating that the other constituents of the herbicide are not inert. There is a compelling need to conduct in vivo studies to characterise the toxicity of glyphosate in a Roundup formulation, to facilitate re-evaluation of existing public health guidelines.

Source.

Read the full study.

 

– See more at: http://healthimpactnews.com/2015/study-roundup-causes-cell-death-to-human-placenta-cells-at-levels-allowed-in-drinking-water/#sthash.AlrycuWi.dpuf

Health Impact News

Australia Determined To Vaccinate By Releast of Aerosolized GMO Vaccine

Australia Determined To Vaccinate By Releast of Aerosolized GMO Vaccine

Dave Mihalovic

Aerial Spraying

© The Viral Post.com

The Office of the Gene Technology Regulator (OGTR) is on its way to approve a licence application from PaxVax Australia (PaxVax) for the intentional release of a GMO vaccine consisting of live bacteria into the environment in Queensland, South Australia, Western Australia and Victoria.

According to the regulator, it qualifies as a limited and controlled release under section 50A of the Gene Technology Act 2000 (the Act).

PaxVax is seeking approval to conduct the clinical trial of a genetically modified live bacterial vaccine against cholera. Once underway the trial is expected to be completed within one year, with trial sites selected from local government areas (LGAs) in Queensland, South Australia, Victoria and Western Australia.

PaxVax has proposed a number of control measures they say will restrict the spread and persistence of the GM vaccine and its introduced genetic material, however there is always a possiblity of these restrictions failing and infecting wildlife and ecosystems.

Aerial vaccines have used in the United States directed towards animals by the use of plastic packets dropped by planes or helicopters.

Sanofi (who is one of the largest vaccine manufacturers in the world) has subsidiary companies such as Merial Limited who manufacture Raboral, an oral live-virus poisonous to humans yet distributed wildlife in the masses.

GMO Vaccine

© The Viral Post.com

In 2006 Michael Greenwood wrote an article for the Yale School of Public Health entitled, “Aerial Spraying Effectively Reduces Incidence of West Nile Virus (WNV) in Humans.”

The article stated that the incidence of human West Nile virus cases can be significantly reduced through large scale aerial spraying that targets adult mosquitoes, according to research by the Yale School of Public Health and the California Department of Public Health.

Under the mandate for aerial spraying for specific vectors that pose a threat to human health, aerial vaccines known as DNA Vaccine Enhancements and Recombinant Vaccine against WNV may be tested or used to “protect” the people from vector infection exposures.

DNA vaccine enhancements specifically use Epstein-Barr viral capsides with multi human complement class II activators to neutralize antibodies. The recombinant vaccines against WNV use Rabbit Beta-globulin or the poly (A) signal of the SV40 virus.

In early studies of DNA vaccines it was found that the negative result studies would go into the category of future developmental research projects in gene therapy.

During the studies of poly (A) signaling of the SV40 for WNV vaccines, it was observed that WNV will lie dormant in individuals who were exposed to chicken pox, thus upon exposure to WNV aerial vaccines the potential for the release of chicken pox virus would cause a greater risk to having adult onset Shingles.

CALIFORNIA AERIAL SPRAYING for WNV and SV40

In February 2009 to present date, aerial spraying for the WNV occurred in major cities within the State of California. During spraying of Anaheim, CA a Caucasian female (age 50) was exposed to heavy spraying, while doing her daily exercise of walking several miles.

Heavy helicopter activity occurred for several days in this area. After spraying, she experienced light headedness, nausea, muscle aches and increased low back pain.

She was evaluated for toxicological mechanisms that were associated with pesticide exposure due to aerial spraying utilizing advanced biological monitoring testing.

The test results which included protein band testing utilizing Protein Coupled Response (PCR) methods were positive for KD-45. KD-45 is the protein band for SV-40 Simian Green Monkey virus.

Additional tests were performed for Epstein-Barr virus capside and Cytomeglia virus which are used in bioengineering for gene delivery systems through viral protein envelope and adenoviral protein envelope technology.

The individual was positive for both; indicating a highly probable exposure to a DNA vaccination delivery system through nasal inhalation.

Pentagon Document Revealed Aerial Vaccination Plans

In the Quarterly FunVax Review in June, 2007, the report lists the objective of a project listed as ID: 149AZ2 as a preparation of a viral vector that will inhibit/decrease the expression of a specific disruption gene (VMAT2) within a human population.

It further indicates in the abstract that six methods of virus dispersal were tested including high altitude release, water supply release, insect transmission, and various methods of diffusion.

Sources:

The Office of the Gene Technology Regulator
VacTruth
Centers for Disease Control and Prevention

Dave Mihalovic is a Naturopathic Doctor who specializes in vaccine research, cancer prevention and a natural approach to treatment.